Purified Uricase

Preparation of uricase.

Uric Acid Analysis with Uricase

Results with two ultramicro methods for plasma uric acid analysis with uricase are described: a crystal violet (gentian violet) method, modified from an automated procedure by Gochman and Schmitz [C/in. Chem. 17, 1154 (1971)] and adapted to manual analysis, and a polarographic oxygen-sensing system (Beckman Instruments,Inc.).The coefficientof variation (CV) for the crystal violet method varied ...

Uricase and its clinical applications

More than three decades ago allopurinol was considered as a first line drug which regulate uric acid synthesis by inhibiting xanthine oxidase (XO) enzyme. . In addition to allopurinol, febuxostat is also used to reduce the synthesis of uric acid by inhibiting the XO enzyme and is approved by the European Medical Agency in 2008 and US FDA in 2009. . In contrast to allopurinol it acts on both red...

The intracellular localization of uricase.

Lan (1, 2) has measured the uricase activity of rat liver homogenate and liver cell nuclei. He found the nuclear activity to be 30 to 46 per cent higher than that of the homogenates wl n both were computed on a dry weight basis. In connection with our studies of purine metabolism we have investigated the intracellular distribution of uricase activity. Differential centrifugation was employed to...

The mechanism of the action of uricase.

1. On the basis of its action on a number of conjugated hexuronic acids and other compounds, it is concluded that mouse-liver glucuronidase is specific for f-D-glucopyranuronides, including ,B-D-glucopyranuronic acid-1-phosphate. 2. Derivatives of klevomenthol caused slight activation of glucuronidase. 3. Mouse-liver preparations did not catalyse the condensation of o-aminophenol with aor ,Bglu...